Abstract
Callus generation from embryo explants of the oil palm (Elaeis guineensis Jacq) was observed in relation to age of plants at time of explants excision and types of media used. The two stages of explants were those at 15 and 18 weeks after anthesis. Eeuwens and Murashige & Skoog‘s (MS) media supplemented with Naphthalene acetic acid (NAA) were used. These gave two media variations viz: MS medium supplemented with NAA and Eeuwens‘ medium supplemented with NAA. The best combination for the generation of callus from the embryo explants within 56 days in culture was determined by observing type of response, time of initiation of callus and callusing explants. It was found that callus generation was best from embryo explants taken from 18 weeks after anthesis cultured in Eeuwens‘ medium, supplemented with NAA. The optimum concentration of NAA was found to be 50 mgl-1, which initiated callus within 39 days in culture. The percentage of explants producing callus was 80 %. The results revealed the importance of the supplementation of nutrient media with NAA and the usefulness of selecting embryo explants of about 18 weeks after anthesis for use in callus generation in the oil palm
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Schedule a callMore From: Nigerian Journal of Life Sciences (ISSN: 2276-7029)
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