Abstract
In vitro, in vivo and clinical studies have demonstrated anti-cancer effects of deuterium depleted water (DDW). The nature of this agents action, cytotoxic or cytostatic, remains to be elucidated. We here aimed to address the point by examining effects on different cell lines. 3-(4, 5-dimethylthiazol- 2-yl)-2, 5-diphenyltetrazolium bromide (MTT) -based cytotoxicity analysis was conducted for human breast, stomach, colon, prostate cancer and glioblastoma multiforme cell lines as well as human dermal fibroblasts. The cell lines were treated with decreasing deuterium concentrations of DDW alone, paclitaxel alone and both. One way analysis of variance (ANOVA) was used for statistical analysis. Treatment with different deuterium concentrations of DDW alone did not impose any significant inhibitory effects on growth of cell lines. Paclitaxel significantly decreased the survival fractions of all cell lines. DDW augmented paclitaxel inhibitory effects on breast, prostate, stomach cancer and glioblastoma cell lines, with influence being more pronounced in breast and prostate cases. DDW per se does not appear to have inhibitory effects on the assessed tumor cell lines as well as normal fibroblasts. As an adjuvant, however, DDW augmented inhibitory effects of paclitaxel and thus it could be considered as an adjuvant to conventional anticancer agents in future trials.
Highlights
Deuterium is the heavier stable isotope of hydrogen1998).Following such a promising body of data, human clinical studies evaluated the effectiveness of depleted water (DDW) on the treatment of different cancers
All cell culture vessels were purchased from BD Biosciences (Franklin Lake, NJ, USA). 3-(4, 5-dimethylthiazol-2yl)-2, 5-diphenyltetrazolium bromide (MTT), dimethyl sulfoxide (DMSO) glycine and paclitaxel were obtained from Sigma-Aldrich (Steinheim, Germany).MDA-MB231(human breast adenocarcinoma), HCT-116, PC-3
The present study aimed to investigate the selective anticancer properties of DDW in a cell line panel including neoplastic (MDA-MB-231, PC-3, AGS, U-87MG, HCT116) and normal (HDF-1) cell lines
Summary
Deuterium is the heavier stable isotope of hydrogen1998) Following such a promising body of data, human clinical studies evaluated the effectiveness of DDW on the treatment of different cancers. A retrospective study showed that administration of DDW as adjuvant to, or as extension of conventional therapy increases the median survival time of patients with breast cancer Somlyai(2004). In vivo and clinical studies have demonstrated anti-cancer effects of deuterium depleted water (DDW). The nature of this agents action, cytotoxic or cytostatic, remains to be elucidated. Results: Treatment with different deuterium concentrations of DDW alone did not impose any significant inhibitory effects on growth of cell lines. DDW augmented inhibitory effects of paclitaxel and it could be considered as an adjuvant to conventional anticancer agents in future trials
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