Abstract
The assembly of poliovirus 14 S particles into empty capsids was studied without cell extract (self-assembly) and in extracts of infected or uninfected HeLa cells. The products were analyzed using monoclonal antibodies specific for N1, N2, or H epitopes. The empty capsids formed in infected cell extract, and only those, possessed N2 epitopes like the procapsids formed in vivo, thus showing that a virally encoded or induced factor determines the antigenicity of the assembly product. Based on this observation, a simple immunological assay for the activity of the morphopoietic factor is presented. This factor is shown to lack serotype specificity.
Full Text 
Sign-in/Register to access full text options
Sign-in/Register to access full text options 
Paper version not known
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
