In Vitro Antiproliferative and Antioxidant Effects of Extracts from Rubus caesius Leaves and Their Quality Evaluation.

Daniel Mirosław Grochowski,Sebastian Granica,Monika Tomczykowa,Paulina Polak,Roman Paduch,Michał Tomczyk,Adrianna Dudek,Małgorzata Pleszczyńska,Adrian Wiater

doi:10.1155/2016/5698685

Abstract

The present study was performed to evaluate the effect of different extracts and subfractions from Rubus caesius leaves on two human colon cancer cell lines obtained from two stages of the disease progression lines HT29 and SW948. Tested samples inhibited the viability of cells, both HT29 and SW948 lines, in a concentration-dependent manner. The most active was the ethyl acetate fraction which, applied at the highest concentration (250 μg/mL), decreased the viability of cells (HT29 and SW948) below 66%. The extracts and subfractions were also investigated for antioxidant activities on DPPH and FRAP assays. All extracts, with the exception of water extract at a dose of 250 μg/mL, almost totally reduced DPPH. The highest Fe3+ ion reduction was shown for the diethyl and ethyl acetate fractions. It was more than 6.5 times higher (at a dose 250 μg/mL) as compared to the control. The LC-MS studies of the analysed preparations showed that all samples contain a wide variety of polyphenolics, among which ellagitannins turned out to be the main constituents with dominant ellagic acid, sanguiin H-6, and flavonol derivatives.

Highlights

Drugs of natural origin have been used throughout history to cure or prevent diseases
Modern phytotherapy is engaged in the production of remedies from materials derived from plants and their use in effective and safe therapy. Their main action could be aimed at three aspects: cytostatic activity, especially when therapy concerns tumour tissue, and antiinflammatory and antioxidative or free radical reduction actions. With all this in mind, we have tried to evaluate the cytotoxic and antioxidant activities of Rubus caesius extracts on two human colon cancer cell lines obtained from two stages of disease progression
In the present work we selected leaves of blackberry R. caesius species traditionally used as a remedy to treat many diseases, among them gastrointestinal bleeding and diarrhoea [1, 11]

Summary

Introduction

Drugs of natural origin have been used throughout history to cure or prevent diseases. With all this in mind, we have tried to evaluate the cytotoxic and antioxidant activities of Rubus caesius extracts on two human colon cancer cell lines obtained from two stages of disease progression. We decided to aim our study at the cytotoxic and reduction activity of R. caesius leaves in human colon carcinoma cells.

Results

Conclusion