IN VITRO ANTIOXIDATIVE PROFILING OF DIFFERENT EXTRACTS OF FAGONIA SCHWEINFURTHII (HADIDI) HADIDI

Abstract

The present study was aimed to evaluate in vitro antioxidant activity of Fagonia schweinfurthii (Hadidi) Hadidi. F. schweinfurthii was successively extracted with different solvents such as petroleum ether, chloroform, ethyl acetate and methanol. The antioxidant activity of F. schweinfurthii extracts was investigated using different assays like 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical scavenging activity, 2,2’-azino-bis(3ethylbenzthiazoline-6-sulfonic acid) (ABTS) diammonium salt radical scavenging activity, hydrogen peroxide (H2O2) scavenging activity, nitric oxide (NO) scavenging activity, ferric reducing power and β-carotene bleaching assay. Total phenolic and flavonoid contents of the extracts were determined by using Folin-Ciocalteu method and aluminium chloride method, respectively in order to evaluate a relationship between the antioxidant activity and the phytochemical constituents. The F. schweinfurthii methanolic extract (FSME) exhibited significantly (p < 0.05) higher ABTS radical, NO radical and H2O2 scavenging activity, reducing power ability and inhibition of β-carotene bleaching. Total phenol and flavonoid contents were highest in the FSME (94.61 ± 1.51 mg GAE/g and 21.52 ± 0.17 mg QE/g) respectively. The present study showed that FSME had more antioxidant power than other extracts. Further studies are necessary to isolate active principles responsible for the overall antioxidant activity of the extracts.