Abstract
PurposeFree radicals are considered as the causative agents of a variety of acute and chronic pathologies. Natural antioxidants have drawn attention of the researchers in recent years for their ability to scavenge free radicals with minimal or even no side effects. This study evaluates the antioxidant capacity of agathisflavone, a naturally occurring biflavonoid by a number of in vitro methods.MethodsAgathisflavone was subjected to DPPH, ABTS, OH and NO radical scavenging assay, reducing potential and inhibition of lipid peroxidation (TBARS) test using trolox as a standard.ResultsAgathisflavone showed concentration-dependent antioxidant activity against all types of free radicals used in this study. The antioxidant capacity, reducing potential and inhibition of lipid peroxidation showed by agathisflavone were comparable to that of trolox.ConclusionAgathisflavone exhibited antioxidant capacity, which suggests considering this biflavonoid for the use in the prevention and/or treatment of diseases precipitated by oxidative stress.
Highlights
Free radicals, both reactive oxygen and nitrogen species (ROS/RNS), produced by partial reduction of oxygen and nitrogen respectively, are inevitable processes of our body [1,2,3]
Chemicals Trolox, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,20-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), thiobarbituric acid (TBA), trichloroacetic acid (TCA), sodium nitroprusside (SNP), 2,20-azobis-2-amidinopropane dihydrochloride (AAPH), 2-deoxyribose and potassium ferricyanide were purchased from SigmaAldrich Co
DPPH radical scavenging Agathisflavone was found to scavenge DPPH radical in a concentration-dependent manner with the highest inhibition observed for the highest concentration of agathisflavone tested (0.928 mM)
Summary
Both reactive oxygen and nitrogen species (ROS/RNS), produced by partial reduction of oxygen and nitrogen respectively, are inevitable processes of our body [1,2,3]. The biflavonoids are structurally dimer of flavonoids connected with each other by a C–C or C–O glycosidic linkage [12] Same as those flavonoids, bioflavonoids have been reported for a number of biological activities proved through in vitro and ex vivo studies, Andrade et al Chemistry Central Journal (2018) 12:75 which include anti-inflammatory [13,14,15], inhibition of cytochrome P 450 enzymes [16], antiviral [17, 18], and neuroprotective activity [19, 20]. Little research was done with the intent of describing the antioxidant property of this compound
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