In vitro antioxidant properties of sulfated polysaccharide from brown marine algae Sargassum tenerrimum

Abstract

ObjectiveIn the present study the physico chemical characteristics, total antioxidant capacity (TAC), reducing power and the free radical scavenging potentials (DPPH radical, ABTS, H2O2 radical) of sulfated polysaccharide from marine brown algae Sargassum tenerrimum was investigated. MethodsThe Sargassum tenerrimum seaweed, which have wide pharmaceutical application, were collected from the coastal region of Mandapam (Lat 09° 17′N, Long 79° 07′E), Tamil Nadu, India and evaluated for In vitro antioxidant properties. ResultsThe extract showed higher percentage of carbohydrate (8.20±1.23%) followed by sulphate (6.6±1.42%) and protein (0.86±0.42%). The free radical scavenging potential was found to be higher in ABTS (70.33±2.33%) followed by DPPH (64.66±2.08%) and H2O2 (61.56±2.05%). the TAC was found to be 62.55± 1.40%. The characterization of sulfated polysaccharide by FT-IR spectrum showed the presence of carboxyl, hydroxyl and sulfate groups. The structure of mobility was assed by agarose gel electrophoresis which showed highest mobility at higher pH values especially in buffer carbonate -bicarbonate (pH 10). The molecular weight of the sulfated polysaccharide was determined by gradient polyacrylamide gel electrophoresis which was found to be 40 kDa. Finally, GC-MS analysis of sulfated polysaccharide from S. tenerrimum exhibited peaks corresponding to Benzenamine (31.67%) and Aminocarb (21.45%). The overall results have established that the sulfated polysaccharide from S. tenerrimum could be used as a promising antioxidant agent. ConclusionPhysico-chemical analysis and elemental analysis of crude seaweed polysaccharide from Sargassum tenerrimum a brown algae elicited the antioxidant activity.