In Vitro Antioxidant Capacity and Neuronal Cell Toxicity of Roots of Ostericum koreanum Maximowicz

Hyo Won Jung,Ramalingam Mahesh,Jun Hong Park,Yong-Ki Park

doi:10.1155/2011/183172

Abstract

Ostericum koreanummaximowicz (Umbelliferae), a medicinal herb in Korean Oriental Medicine, has been applied to treat cold, headache, neuralgia and arthralgia. The ethyl acetate fraction ofO. koreanumroot was subjected toin vitroantioxidant activity with different methods for free radical scavenging activities. In addition, the cell viability and nitric oxide release assays were performed here for the first time in neuroblastoma (Neuro-2a) cell cultures. Among all the tested methods, the ethyl acetate fraction was expressed very active, exhibiting a good Trolox equivalent values and IC50, comparable to that of the commercial antioxidants, Trolox and ascorbic acid, respectively. The results showed that there was a reduction of cell viability by the fraction in a concentration dependent manner. These results suggest thatO. koreanumshows good antioxidant activitiesin vitroby inhibiting free radicals. These findings provide a rationale for thein vivotesting. Also, the major constituents behind the antioxidant mechanisms of this fraction warrant further study.

Highlights

Ostericum koreanum maximowicz (Umbelliferae) is used traditionally in Korean Oriental Medicine
The O. koreanum roots ethyl acetate fraction showed potent antioxidant activity based on Trolox equivalent antioxidant capacity (TEAC), oxygen radical absorbance capacity (ORAC) and DPPH and other free radical scavenging tests in a dose dependent manner
The O. koreanum roots were found to be active in TEAC, ORAC and DPPH as 0.516, 2.884 and 1.514 mmole Trolox equivalents per grams, respectively (Table 1)

Summary

Introduction

Ostericum koreanum maximowicz (Umbelliferae) is used traditionally in Korean Oriental Medicine. The most active ethyl acetate fraction was used for bioassays. In vitro antioxidant activity was studied by TEAC11,12, ORAC13, DPPH14, superoxide radical[15], hydrogen peroxide decomposition[16,17], hydroxyl radical[18], nitric oxide radical inhibition[19,20] and metal chelation[21].

Results

Conclusion