Abstract
Objective: In vitro antioxidant activity, in vivo antidiabetic property and intestinal attachment by two potential probiotic bacterial strains, namely, Enterococcus faecium and Enterococcus hirae were studied using albino rats.
 Methods: Antioxidant the activity was assessed using 2,2-Diphenyl-1-picrylhydrazyl radicals scavenging assay. Alloxan was administered intraperitoneally to induce diabetic conditions in experimental rats. Animals were treated with oral administration of Enterococcus spp., such as E. faecium, and E. hirae isolated from goat and sheep milk. The control animal group received normal saline for the same days. Glibenclamide drug was used as a positive control against probiotic bacterial cells.
 Results: However, administration of probiotic bacterial strains E. faecium and E. hirae, in albino rats significantly (p<0.05) at varying doses lowered blood glucose levels in diabetic rats as compared to the diabetic control group. Both the species of Enterococcus increased the bodyweight of experimental rats. However, E. faecium was the best antidiabetic strain having the antioxidant activities also in comparison to E. hirae. The attachment of probiotic bacterial cells E. faecium on the rat’s intestine wall against pathogens was examined. Furthermore, E. faecium showed its aggregation with pathogens by attachment of the intestines of albino rats. This showed that both the bacterial strains exhibited in vivo antidiabetic effect.
 Conclusion: The results of this study showed that probiotic bacteria possess antioxidant, antidiabetic activities, and attachment of intestine.
Highlights
Diabetes is one of the common metabolic disorders throughout the world
The results of this study showed that probiotic bacteria possess antioxidant, antidiabetic activities, and attachment of intestine
The antioxidant activity of E. faecium and E. hirae was measured by DPPH radical scavenging
Summary
Diabetes is one of the common metabolic disorders throughout the world. Diabetes occurs when the body is unable to produce enough insulin or has complications of its proper use [1]. Defects in reactive oxygen species (ROS) scavenging enzymes, deficiencies or disruptions in insulin secretion, and high oxidative stress damaging pancreatic beta cells will result in chronic hyperglycemia and troublesome disruptions in carbohydrate, fat, and protein metabolisms [2]. The beta-pancreas cells are responsible for insulin production in the human body. The damage of β-cells is caused by an increase of ROS through the apoptosis-induction and insulin-biosynthesis suppression [5]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
