In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed Extract.

Deepti Dabas,Ryan J Elias,Gregory R Ziegler,Joshua D Lambert

doi:10.1155/2019/6509421

Deepti Dabas, Ryan J Elias + Show 2 more

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https://doi.org/10.1155/2019/6509421

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Abstract

Avocado (Persea americana) seeds have been used traditionally for a number of health-related indications. Because of its high polyphenol content, we investigated the potential antioxidant and anticancer effects of a colored avocado seed extract (CASE). CASE exhibited an oxygen radical acceptance capacity value of 2012 ± 300 trolox equivalents/mg. CASE reduced lipid hydroperoxide formation in an oil-in-water emulsion (33% reduction at 500 μg/mL). CASE dose-dependently reduced the viability of human breast (MCF7), lung (H1299), colon (HT29), and prostate (LNCaP) cancer cells in vitro. The half maximal inhibitory concentrations ranged from 19 to 132 μg/mL after treatment for 48 h. CASE treatment downregulated the expression of cyclin D1 and E2 in LNCaP cells. This was associated with cell G0/G1 phase cycle arrest. CASE also dose-dependently induced apoptosis in LNCaP cells. CASE reduced nuclear translocation of nuclear factor κB, a prosurvival signal. Further studies are needed to examine these effects in in vivo models.

Highlights

Avocado (Persea americana, Lauraceae) is an important tropical crop that is rich in unsaturated fatty acids, fiber, vitamins B and E, and other nutrients
We have previously reported that when the avocado seed is crushed, a stable orange color develops, and we have investigated the potential use of this colored avocado seed extract (CASE) as a food color additive
We have previously reported that CASE contains high levels of polyphenols and in the current study we explored the potential antioxidant and anticancer activity of the extract using in vitro models (Dabas et al, 2011)

Summary

Introduction

Avocado (Persea americana, Lauraceae) is an important tropical crop that is rich in unsaturated fatty acids, fiber, vitamins B and E, and other nutrients. The Hass avocado is the most important variety grown commercially. In 2016, Mexico, the world’s largest grower, produced 1.9 million metric tons of Hass avocados, while the U.S produced 172,630 metric tons (http://www.fao.org). Ethnopharmacological studies of the Aztec and Maya cultures have reported the use of decoctions of avocado seeds for the treatment of mycotic and parasitic infections, diabetes, inflammation, and gastrointestinal irregularity. Our previous review highlights multiple potential applications of avocado seeds including insecticidal, antimicrobial, antidiabetic, and blood pressure reducing effects [2]

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