Abstract
Objective: The objective of this study was to evaluate the antioxidant and anti-inflammatory activities of the solid powder obtained from the ethyl acetate fraction from the flower Opuntia stricta.
 Methods: The flower extract was evaluated for antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay and reducing power assay was carried out by ferric-reducing capacity (FRC) assay method. The in vitro anti-inflammatory activity was evaluated using human peripheral blood mononuclear cells (PBMCs) stimulated by lipopolysaccharide (LPS) to evaluate nitric oxide (NO) production method.
 Results: The solid powder obtained from the ethyl acetate fraction from the flower O. stricta showed a good antioxidant activity in scavenging DPPH radical and FRC assay with compared standard sample. This solid powder also showed good anti-inflammatory activity in cell viability (LPS-induced PBMCs) assay and NO assay.
 Conclusion: These results suggest that the solid powder obtained from the ethyl acetate fraction from the flower O. stricta has significant antioxidant and anti-inflammatory activities.
Highlights
Numerous higher plants are real wellsprings of regular items utilized as pharmaceuticals, agrochemicals, flavor and scent fixings, sustenance added substances, and pesticides [1]
DPPH is usually used to test the capacity of mixes to go about as free extreme foragers or hydrogen contributors and it depends on the capacity of DPPH to decolorize within the sight of antioxidant [31,32]
The inhibition of the DPPH radical by the solid powder obtained from the ethyl acetate fraction from the flower O. stricta was concentration dependent (Fig. 1)
Summary
Numerous higher plants are real wellsprings of regular items utilized as pharmaceuticals, agrochemicals, flavor and scent fixings, sustenance added substances, and pesticides [1]. Uncontrolled era of free radicals prompts assault on layer lipids, proteins, catalysts, and DNA causing oxidative anxiety and at last cell demise. These reactive oxygen species (ROS) are dependable for some degenerative human illnesses such as diabetes mellitus, tumor, neurodegenerative scatters, Alzheimer’s malady, Parkinson’s ailment, atherosclerosis, maturing, and provocative diseases [5]. There are different chemical frameworks with the expectation of complimentary radical rummaging yet micronutrients such as Vitamin E, beta-carotene, and Vitamin C are the significant antioxidant. These must be given in consume less calories as body cannot deliver these nutrients [7]. Antioxidants from plant materials diminish the activity of free radicals and shield our body from different infection [8,9]
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