Abstract
The aim of our present study was to evaluate the antioxidant and anti-lipid peroxidation activity of ethanolic extracts of <em>Bougainvillea species viz B. shubhra, B. peruviana and B. bhuttiana</em> golden glow. Phytochemical constituents viz. total phenolics, flavonoids and tannins were assayed using standard protocol. The antioxidant activity was studied by DPPH assay, FRAP assay, reducing power assay and in-vitro inhibition of lipid peroxidation. Of the three species, <em>B. bhuttiana</em> golden glow showed highest amount of phenols (6.78±0.001), flavonoids (27.7±0.012) and tannin (11.08±0.008) contents. It also showed highest antioxidant activity of 15.15± 0.008 and 8.08± 0.018 as determined by FRAP assay and reducing power assay respectively. The percent DPPH radical scavenging activity was 82.72% and in-vitro inhibition of lipid peroxidation in mitochondrial membrane was also found to be highest in <em>B. bhuttiana</em> golden glow.
Highlights
(INH) and Pentylenetetrazole (PTZ) induced seizures and significantly inhibited the attenuation of brain Gamma-Amino Butyric Acid (GABA) levels
These results suggested that the observed beneficial effect in epilepsy may be by enhancing the GABAergic system
The extent of lipid peroxidation in rat liver mitochondria was assayed by estimating thiobarbituric acid-reactive substances (TBARS) by standard assay [20]
Summary
Oven dried, ground to fine powder and stored in airtight containers until further use. 20gm of dried powder was defatted with petroleum ether using soxhlet apparatus followed by successive solvent extraction using acetone, ethanol and distilled water [9]. Oven dried, ground to fine powder and stored in airtight containers until further use. 20gm of dried powder was defatted with petroleum ether using soxhlet apparatus followed by successive solvent extraction using acetone, ethanol and distilled water [9]. 300 ml volume of each solvent and 15 cycles of extraction were followed. The resulting fractions were concentrated using rotary evaporator, final residue was reconstituted in DMSO and stored at -20°C. This crude extract was suitably diluted and assayed for phytochemical and antioxidant activity
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