Abstract

The aim of this study was to contribute to the valorisation of the plant by determining the antioxidant activity of Stereospermum kunthianum trunk bark hydroethanolic extract and its fractions obtained with solvents of increasing polarity. Antioxidant activity was screened by bioautography and assessed by DPPH, ABTS and FRAP assays. All samples shown an antioxidant activity by the bioautography test. The ethyl acetate fraction had shown better activity in DPPH and ABTS assays with CI50 of 4.8 ± 0.24 μg/ml and 78.6 ± 0.38 μg/ml respectively. In the FRAP method, the ethyl acetate fraction shown also better activity with a percentage reduction close to that of the ascorbic acid (94.44 ± 0.25% and 96.01 ± 0.04% respectively, at the highest concentration (10.68 μg/ml). Trunk bark hydroethanolic extract of Stereospermum kunthianum possess an antioxidant activity which increase significatively after fractionation with ethyl acetate and butanol.

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