Abstract
Hyperglycemia induced oxidative stress is one of the main mechanism involved in the pathogenesis of diabetic complications. Use of antioxidants as an adjuvant therapeutic approach in diabetes could prevent or delay the progression of oxidative stress induced diabetic complications. Many herbal drugs possess multiple pharmacological actions like anti-hyperglycemic, anti-obesity and anti-oxidant properties etc., which could act through multiple pathways and may offer beneficial effect in the management of multi-factorial diseases such as diabetes. Development of herbal formulation with ingredients having multiple actions could be a potential approach for improving therapeutic efficacy. We here report the free radical scavenging activity of a herbal mixture, DIA-2 and its individual herbs. DIA-2, a herbal formulation containing fixed combination of standardized aqueous extracts of Allium sativum (ASE) bulbs and Lagerstroemia speciosa (LSE) leaves. The present study was undertaken to investigate whether a combination of ASE and LSE shows a synergistic antioxidant effect than its individual herbs. In vitro antioxidant activity for individual herbs and DIA-2 was determined by DPPH radical scavenging, nitric oxide scavenging assay, total antioxidant capacity, reducing power assay and ABTS radical scavenging methods respectively. Our results suggest that DIA-2 could synergistically enhance the antioxidant activity and could be attributed due to the synergistic actions of the individual ingredients. The data obtained suggests the combined use of ASE and LSE as active ingredients in the development of antidiabetic herbal formulation, synergizing its therapeutic value in treating hyperglycemia and associated oxidative stress.
Highlights
Hyperglycemia is the main causative factor for oxidative stress during diabetes mellitus (DM)
In vitro antioxidant activity for individual herbs and DIA-2 was determined by DPPH radical scavenging, nitric oxide scavenging assay, total antioxidant capacity, reducing power assay and ABTS radical scavenging methods respectively
Our results suggest that DIA-2 could synergistically enhance the antioxidant activity and could be attributed due to the synergistic actions of the individual ingredients
Summary
Hyperglycemia is the main causative factor for oxidative stress during diabetes mellitus (DM). Recent research studies indicate that current treatments available for the current research and development on drugs has targeted on oxidative stress pathways for the prevention of diabetic complications (Omar et al, 2010). Use of antioxidants is considered as one of the therapeutic remedy for the prevention of diabetic complications till date (Xie et al, 2009). Use of synthetic antioxidants is not helpful and has been reported for their adverse effects (Radulović et al, 2007). Plants based medicines have been found to possess excellent antioxidant activities and used in combination to demonstrate significant therapeutic effects than when used alone (Yang et al, 2009)
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