In-vitro antioxidant activity of hydroalcoholic extract of crinum latifolium using different method

Abstract

The purpose of this research is to look for phytochemicals and determine the antioxidant potential of C. latifolium aerial parts. The qualitative analysis of several phytochemical components was determined using a well-known test methodology. The 1,1-diphenyl-2-picryl hydrazyl (DPPH) assay, hydrogen peroxide, and nitric oxide radical scavenging method were used to investigate the antioxidant activity of a hydro ethanol extract of Crinum latifolium bark in vitro. Phytochemical studies discovered the existence of phenols and flavonoids. The extract demonstrated dose-dependent free radical scavenging capabilities in the models studied. For the DPPH method, aerial portions of Crinum latifolium extract had an IC50 value of 46.35µg/ml, which was close to that of ascorbic acid (IC50=20.03g/ml). The IC50 value for the Superoxide radical scavenging assay was determined to be 60.94µg/ml, which is comparable to ascorbic acid (IC50= 20.91g/ml). The IC50 value was determined to be 50.30µg/ml in reducing power determination, which was close to that of ascorbic acid (IC50=20.84g/ml). The phytochemical profile and antioxidant activity of Crinum latifolium, which will be exploited for medicinal purposes, are described in this paper.