In vitro antioxidant activity evaluation and HPTLC profile of Cow dung

Abstract

Aim: The present study was undertaken to evolve suitable High Performance Thin Layer Chromatography (HPTLC) profile and to evaluate in vitro antioxidant activity of cow dung. Materials and Methods: Dung samples, collected from cows of Geer variety of Bos indicus on different days at predefined times, were subjected to development of HPTLC profile, estimation of total phenolic content and in vitro antioxidant activity by HPTLC‑2, 2 Diphenyl‑1‑picrylhydrazil (DPPH) bioautography, Ferric Reducing Antioxidant Power (FRAP) and DPPH free radical scavenging methods. Results: Developed HPTLC profile revealed notable variations among the samples. HPTLC‑DPPH Bioautography showed presence of number of antioxidant compounds, which was further confirmed by quantitative evaluation of in vitro antioxidant activity. The results of the study indicate presence of phenolic as well as non‑phenolic natured bio‑active compounds in cow dung. Conclusion: The HPTLC profile as well as antioxidant potential of cow dung is reported for the first time with notable intra‑day and inter‑day variations. The evolved chromatographic profile will be very useful for evaluating the quality of cow dung. It has potential as a natural antioxidant. Key words: Antioxidant, cow dung, high performance thin layer chromatography, HPTLC‑2, 2 Diphenyl‑1‑picrylhydrazil bioautography