Abstract

Objective: To investigate the antioxidant activity of ethanolic extract of Terminalia bellerica fruit pulp (TBFP), seed (TBS) and bark (TBB), and identification of phytochemical constituents of said extracts.Methods: The antioxidant potential of T. bellerica (TB) parts was evaluated by free radical scavenging activity (FRSA), superoxide anion radical scavenging activity (SARSA), hydroxyl radical scavenging activity (HRSA) and compared with reference standard quercetin. Lipid peroxidation (LPO), ferric thiocyanate activity (FTC) and reducing power (RP) of the plant extracts were also examined. The phytochemical constituents of said extracts have been quantified by gas chromatography-mass spectrometer (GC-MS) method.Results: Ethanolic extract of TBFP exhibited high phenolic content (254.33) followed by TBS (227.33) and TBB (185) mg/g of GAE. TBFP showed lowest IC50 for FRSA (62 µg/ml), SARSA (39 µg/ml) and HRSA (27 µg/ml) and highest RP (3.39 ascorbic acid equivalents/ml). TBB showed lowest IC50 for LPO (48 µg/ml) whereas TBS showed lowest IC50 for FTC (109 µg/ml). Predominant phyto-compounds present in TB extracts were quinic acid, gallic acid, ethyl galate, 9, 12 octadecadienoic acid and glucopyranose in varying concentrations as analyzed by GC-MS.Conclusion: On the basis of correlation of antioxidant studies with total phenolic content (TPC) and GC-MS analysis of different part of TB, it may be concluded that high concentration of total phenolics and other bioactive phytochemicals of TB extracts make it a potential source of nutraceutical antioxidants.

Highlights

  • Terminalia bellerica (TB) Roxb (Combretaceae), is a large deciduous tree and is commonly known as beleric mycobalane

  • The results revealed that plant extracts containing higher phenolic compounds showed maximum antioxidant activity

  • gas chromatography-mass spectrometer (GC-MS) analysis revealed the presence of various bioactive constituents in ethanolic extracts of TB plant parts which might be responsible for their antioxidant activity

Read more

Summary

Introduction

Terminalia bellerica (TB) Roxb (Combretaceae), is a large deciduous tree and is commonly known as beleric mycobalane It has been valued in Ayurvedic and traditional system of medicine for treatment of wide range of diseases having many pharmacological properties such as immunomodulatory, anti-inflammatory, anticancer, hepatoprotective and antimicrobial activities [1,2,3,4,5]. It is an integral part of the traditional laxative formulation, Triphala which is used for a variety of ailments in Ayurvedic medicines since ancient times. TB enhances the body resistance against diseases and is used as herbal medicine to get remedies from all the above ailments [9]

Methods
Results
Discussion
Conclusion
Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call