Abstract
Schizochytrium protein hydrolysate (SPH) was prepared through stepwise enzymatic hydrolysis by alcalase and flavourzyme sequentially. The proportion of hydrophobic amino acids of SPH was 34.71%. The molecular weight (MW) of SPH was principally concentrated at 180–3000 Da (52.29%). SPH was divided into two fractions by ultrafiltration: SPH-I (MW < 3 kDa) and SPH-II (MW > 3 kDa). Besides showing lipid peroxidation inhibitory activity in vitro, SPH-I exhibited high DPPH and ABTS radicals scavenging activities with IC50 of 350 μg/mL and 17.5 μg/mL, respectively. In addition, the antioxidant activity of SPH-I was estimated in vivo using the model of acute alcohol-induced liver injury in mice. For the hepatoprotective effects, oral administration of SPH-I at different concentrations (100, 300 mg/kg BW) to the mice subjected to alcohol significantly decreased serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities and hepatic malondialdehyde (MDA) level compared to the untreated mice. Besides, SPH-I could effectively restore the hepatic superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities and glutathione (GSH) level. Results suggested that SPH was rich in biopeptides that could be exploited as antioxidant molecules against oxidative stress in human body.
Highlights
Schizochytrium sp., a kind of heterotrophic marine fungus, is well known for the production ofΩ-3 fatty acids, pigments, proteins, polysaccharides, etc. [1,2]
The present study suggests that Schizochytrium protein hydrolysates have the potential in increasing resistivity against oxidative stress in the human body
Udenigwe et al [14] indicated that acidic amino acids such as Glu and Asp contributed to the antioxidant activities of peptides due to the presence of excess electrons which could be donated during interaction with free radicals
Summary
Schizochytrium sp., a kind of heterotrophic marine fungus, is well known for the production ofΩ-3 fatty acids, pigments, proteins, polysaccharides, etc. [1,2]. Schizochytrium sp., a kind of heterotrophic marine fungus, is well known for the production of. Ω-3 fatty acids, pigments, proteins, polysaccharides, etc. A number of researchers have focused on the industrial production of docosahexaenoic acid for Schizochytrium sp. There is little information on the utilization of Schizochytrium sp. In addition to a high content of fat, Schizochytrium sp. Contains a high amount of protein, which is about 40% (dry weight). Great efforts are needed to transform these biological wastes into value-added bioproducts. The utilization of protein recovered from the defatted byproduct presents an opportunity to develop pharmaceutical products and food ingredients
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