Abstract
This study evaluated the in vitro antineoplastic and antiviral potential and in vivo toxicity of twelve extracts with different polarity obtained from the herbaceous perennial plant Geum urbanum L. (Rosaceae). In vitro cytotoxicity was determined by ISO 10993-5/2009 on bladder cancer, (T-24 and BC-3C), liver carcinoma (HEP-G2) and normal embryonic kidney (HEK-293) cell lines. The antineoplastic activity was elucidated through assays of cell clonogenicity, apoptosis induction, nuclear factor kappa B p65 (NFκB p65) activation and total glutathione levels. Neutral red uptake study was applied for antiviral activity. The most promising G. urbanum extract was analyzed by UHPLC–HRMS. The acute in vivo toxicity analysis was carried out following OEDC 423. The ethyl acetate extract of aerial parts (EtOAc-AP) exhibited the strongest antineoplastic activity on bladder cancer cell lines (IC50 = 21.33–25.28 µg/mL) by inducing apoptosis and inhibiting NFκB p65 and cell clonogenicity. EtOAc and n-butanol extracts showed moderate antiviral activity against human adenovirus type 5 and human simplex virus type I. Seventy four secondary metabolites (gallic and ellagic acid derivatives, phenolic acids, flavonoids, etc.) were identified in EtOAc-AP by UHPLC–HRMS. This extract induced no signs of acute toxicity in liver and kidney specimens of H-albino mice in doses up to 210 mg/kg. In conclusion, our study contributes substantially to the detailed pharmacological characterization of G. urbanum, thus helping the development of health-promoting phytopreparations.
Highlights
Geum urbanum L. is a herbaceous perennial plant species, commonly known as wood avens or St
We chose the cell lines HEK293 and HEP-G2 for preliminarily evaluating the in vitro cytotoxicity of the investigated extract as a first step before performing the acute toxicity study, taking into consideration that the liver and kidney are the most important organs involved in the metabolism and excretion of xenobiotics after per oral administration
A preliminary test for antineoplastic activity was performed on the human tumor cell line T-24 originating from human bladder carcinoma, stage III
Summary
Geum urbanum L. (genus Geum, family Rosaceae) is a herbaceous perennial plant species, commonly known as wood avens or St. (genus Geum, family Rosaceae) is a herbaceous perennial plant species, commonly known as wood avens or St. Benedict’s herb, and widely spread in the temperate regions of Europe [1,2], e.g., Bulgaria. Benedict’s herb, and widely spread in the temperate regions of Europe [1,2], e.g., Bulgaria It was used from ancient times in the European and in the Bulgarian traditional medicine for gastro-intestinal disorders to reduce bleeding and the inflammation of mucous membranes [3,4,5]. The species is listed in the VOLKSMED database as a traditional Austrian herb with anti-inflammatory activity [6].
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