In vitro antimycotic activity of a new isolate Streptomyces fradiae MTCC 11051 against the multi-drug resistant pathogenic fungi

Abstract

BackgroundIsolation of naturally occurring Streptomyces sp. with an ability to produce metabolites having antimycotic property against the pathogenic fungus from unexplored area of Chhattisgarh. MethodsStarch casein nitrate medium was used for isolation and screened for their antifungal activity by agar well diffusion method. Active isolate characterized based on the morphological, physiological and 16S rRNA sequencing. Optimization of extracellular antifungal metabolite production in terms of zone of inhibition (mm) using Candida albicans MTCC 183 as target organisms was performed and extracted with n-butanol. The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of active metabolite was determined according to NCCLS. Results and discussionThe active isolate was identified as Streptomyces fradiae MTCC 11051, showed the best antifungal activity against yeasts and molds including dermatophytes which showed an inimitable and stable inhibitory activity in Starch casein nitrate broth. The maximum extracellular active metabolite production was achieved in the late log phase, which remained constant during the stationary phase. The MIC and MFC values of the culture supernatant were recorded between range 50 and 100 μg/ml. ConclusionsThe metabolite from S. fradiae was active against pathogenic fungus with a greater potency. These results confirmed the therapeutic potential of bioactive fermented products can be used in medicinal and pharmaceutical preparations for treating the fungal infections.