Abstract
The in vitro activities of enrofloxacin, florfenicol, oxytetracycline and spiramycin were determined against field isolates of Mycoplasma mycoides mycoides large colony (MmmLC) by means of the broth microdilution technique. The minimum inhibitory concentrations (MICs) of these antimicrobial drugs were determined for a representative number of 10 isolates and 1 type strain. The susceptibility of Arcanobacterium pyogenes to enrofloxacin, oxytetracycline and tilmicosin was determined by means of an agar disk diffusion test. The MICs of enrofloxacin, florfenicol, oxytetracycline and spiramycin were within the ranges of 0.125-0.5, 1.0-2.0, 2.0-4.0 and 4.0-8.0 microg/ml, respectively. This study has shown that resistance of MmmLC against enrofloxacin, florfenicol, oxytetracycline and spiramycin was negligible. All the field strains of A. pyogenes that were tested were susceptible to enrofloxacin, oxytetracycline and tilmicosin with mean inhibition zones of 30.6, 42.3 and 35.8 mm, respectively. Although there is lack of data on in vivo efficacy and in vitro MIC or inhibition zone diameter breakpoints of these antimicrobial drugs for MmmLC, the MIC results indicate that these 4 classes of antimicrobial drugs should be effective in the treatment of ulcerative balanitis and vulvitis in sheep in South Africa.
Highlights
Ulcerative balanoposthitis and vulvovaginitis of sheep is a venereal disease characterised by erosion and ulceration of the glans penis and vulval labia
The objective of this study was to determine the in vitro sensitivity of Mycoplasma mycoides mycoides large colony (MmmLC) and A. pyogenes isolated from field cases of ulcerative balanitis and vulvitis (UBV) to a variety of these drugs in order to assist in the selection of those most appropriate for the treatment of clinical cases
The minimum inhibitory concentrations of the antibiotics to which the MmmLC field isolates were susceptible are shown in Tables 3 and 4
Summary
Ulcerative balanoposthitis and vulvovaginitis of sheep is a venereal disease characterised by erosion and ulceration of the glans penis and vulval labia. It has been described in several countries[6,11,14,19,20,29,30] and has been recognised in South Africa since 197929,30 where sheep of the Dorper breed appear to be the most frequently affected[6,15,29]. Various names have been given to the syndrome or similar syndromes. These aNational Animal Health Research Centre, PO Box 04, Sebeta, Ethiopia
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