Abstract
Abstract Purpose To demonstrate the in vitro bactericidal, bacteriostatic or inert role of endotamponading substances. Methods Clinical isolates of Staphylococcus epidermidis, Staphylococcus aureus and Clostridium sp were cultured on blood agar plates in sulphur hexafluoride (SF6)and perfluoropropane (C3F8)atmospheres and under silicone oil (SO) and liquid perfluorocarbon (LPFC). The same germs were cultured under aerobic and anaerobic atmospheres as controls. Results SO, SF6 and C3F8 did not significantly inhibit the growth of anaerobic bacteria but markedly reduced the growth of aerobic germs, as compared with aerobic conditions. The growth of the bacteria under SO and SF6 and C3F8 was very similar to that observed under anaerobic conditions. LPFC did not affect the growth of aerobic or anaerobic bacteria. Conclusion Endotamponading gases and liquids seem to improve the visual and anatomical outcome of infected eyes and may limit the proliferation of aerobic germs in vitro. It is not known to what extent the antimicrobial effect of these substances would appear in vivo since oxygen diffusion from the blood could allow the growth of aerobic germs.
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