Abstract
Background and Purpose:Root canal therapy is the primary method for the treatment of an infected pulp in modern dentistry. The main aim of endodontic treatment is the elimination of bacteria and their products from infected root canals. In this study, we attempted to investigate the antimicrobial activity of three root canal sealers against oral pathogens.Materials and Methods:The antimicrobial effectiveness of three endodontic sealers with different chemical compositions, namely resin (AH 26), zinc oxide eugenol (ZOE), and mineral trioxide aggregate (MTA), against Candida albicans, Streptococcus sanguis, Streptococcus salivarius, Streptococcus mutans, and Lactobacillus casei was assayed by agar well diffusion method (AWDM). The tested sealers were prepared according to the manufacturer’s instructions and poured in the prepared wells of agar plates; diluted inocula (105 and 106 CFU/ml) of the tested microorganism strains were also used. The minimum inhibitory concentration (MIC) values of the selected canal sealers ranged between 3.12 and 50 mg.ml-1 against the employed microorganism strains. All the plates were incubated at 37°C under anaerobic condition for bacteria and at 30°C for C. albicans. After three days, the inhibition zones were measured. Results:In this investigation, AH 26 exhibited strong activity against C. albicans with the minimum inhibitory concentration of 12.5 mg.ml-1, but ZOE and MTA did not act against C. albicans. ZOE sealer had the highest antimicrobial activity against the tested bacteria, while MTA showed the lowest antimicrobial activity. Conclusion:The ascending sequence of microbial growth inhibition zones was as follows AH 26 > ZOE > MTA.
Highlights
Microbes and their products are the most common etiologic agents of pulpitis and apical periodontitis [1,2]
We investigated the effects of different root canal sealers including AH 26, zinc oxide eugenol (ZOE), and mineral trioxide aggregate (MTA) on five types of isolated oral pathogenic microorganisms (i.e., C. albicans, S. sanguis, S. salivarius, S. mutans, and L. casei)
The antimicrobial efficacy of each endodontic sealer was measured by the diameter of the inhibition zone around each well filled by a sealer for each kind of bacterium and fungus
Summary
Microbes and their products are the most common etiologic agents of pulpitis and apical periodontitis [1,2]. One of the main causes of root canal treatment failure is the presence of facultative and resistant microbial species of the oral cavity, namely C. albicans, Enterococcus faecalis, Staphylococcus aureus, and Streptococcus spp. There is some evidence as to the antimicrobial activity of root canal sealers such as mineral trioxide aggregate (MTA), zinc oxide eugenol (ZOE), and resin (AH 26). We attempted to investigate the antimicrobial activity of three root canal sealers against oral pathogens. Materials and Methods: The antimicrobial effectiveness of three endodontic sealers with different chemical compositions, namely resin (AH 26), zinc oxide eugenol (ZOE), and mineral trioxide aggregate (MTA), against Candida albicans, Streptococcus sanguis, Streptococcus salivarius, Streptococcus mutans, and Lactobacillus casei was assayed by agar well diffusion method (AWDM). Conclusion: The ascending sequence of microbial growth inhibition zones was as follows AH 26 > ZOE > MTA
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