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Abstract
BackgroundActinomycetes are Gram-positive, often filamentous, bacteria known for their unsurpassed capacity for the production of secondary metabolites with diverse biological activities. The aim of the present study was to evaluate the antimicrobial, cytotoxic and antioxidant properties of Streptomyces lavendulae strain SCA5.ResultsThe ethyl acetate extract of SCA5 broth (EA-SCA5) showed antimicrobial activity with MIC value of 31.25 μg/ml. EA-SCA5 showed good antioxidant potential by scavenging 2, 2-diphenyl-picrylhydrazyl (DPPH) (IC50 507.61 ± 0.66 μg/ml), hydroxyl radical (IC50 617.84 ± 0.57 μg/ml), nitric oxide (IC50 730.92 ± 0.81 μg/ml) and superoxide anion radical (IC50 864.71 ± 1.15 μg/ml). The EA-SCA5 also showed strong suppressive effect on rat liver lipid peroxidation (IC50 838.83 ± 1.18 μg/ml). The total phenolic content of SCA5 was 577.12 mg of GAE equivalents/gram extract. EA-SCA5 exhibited cytotoxic activity on A549 adenocarcinoma lung cancer cell line. It showed 84.9% activity at 500 μg/ml with IC50 value of 200 μg/ml. The gas chromatography mass spectrometry (GC-MS) analysis revealed the presence of one major bioactive compound actinomycin C2.ConclusionsThe results of this study indicate that the EA-SCA5 could be probed further for isolating some medically useful compounds.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-014-0291-6) contains supplementary material, which is available to authorized users.
Highlights
Actinomycetes are Gram-positive, often filamentous, bacteria known for their unsurpassed capacity for the production of secondary metabolites with diverse biological activities
The reference bacterial cultures were obtained from the Institute of Microbial Technology (IMTECH), Chandigarh, India-160 036 and all the fungal cultures were obtained from the Department of Microbiology, Christian Medical College, Vellore, Tamil Nadu, India
SCA5 strain was identified by morphological, biochemical, physiological characteristics and 16S rRNA amplification
Summary
Actinomycetes are Gram-positive, often filamentous, bacteria known for their unsurpassed capacity for the production of secondary metabolites with diverse biological activities. The aim of the present study was to evaluate the antimicrobial, cytotoxic and antioxidant properties of Streptomyces lavendulae strain SCA5. Free radicals are known to be the major cause of various chronic and degenerative diseases, including aging, coronary heart disease, inflammation, stroke, diabetes mellitus and cancer. Oxidative stress occurs when there is excessive free radical production and/or low antioxidant defence, which leads to chemical alterations of biomolecules causing structural and functional modifications [2]. The antimicrobial, antioxidant and cytotoxic properties of extracellular crude extracts isolated from the fermented broth of soil-associated Streptomyces lavendulae strain SCA5 were investigated
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