In-vitro Antimicrobial Activity of Mtad, Photoactivated Disinfection Along with Chlorohexidine on Enterococcus faecalis, Staphylococcus aureus and Candida albicans

Abstract

This study evaluated the antimicrobial activity of five root canal irrigants against MTCC of Enterococcus faecalis, Staphylococcus aureus, Candida albicans, in-vitro study of the inhibitory activity of PAD in conventional Endodontic Treatment (CET) and also combination of CET with PAD (CHX+PAD). All of these microorganisms were incubated in the presence of an Endodontic irrigant. Agar diffusion tests were performed and colony counts were tested using paper point to evaluate the inhibitory effect of the irrigants. The mean diameters of the inhibition zones for C. albican were 21mm 3% Naocl, 19mm CHX, 0.7PAD, 22mm CHX+PAD, 20mm Fluconazole, 18mm Doxycycline. The zone diameters of the inhibition zones of Enterococcus faecalis were 17mm 0.5% Naocl, 18mm CHX, 12mmPAD, 35mm MTAD, and 19mm PAD+CHX. The Zone Diameter of the inhibition zones of Staphylococcus aureus were 18mmCHX, 12mm PAD, 19mm PAD+CHX, 25mm MTAD, and 19mm 3% NaoCl. Together High inhibitory effect was detected for PAD+CHX and MTAD on all three pathogens. Candida showed more inhibition to all irrigants followed by Staphylococcus aureus and Enterococcus faecalis.