In vitro antimicrobial activity of a novel compound, Mul-1867, against clinically important bacteria.

George Tetz,Victor Tetz

doi:10.1186/s13756-015-0088-x

Abstract

BackgroundThe antimicrobial activity of Mul-1867, a novel synthetic compound, was tested against 18 bacterial strains, including clinical isolates and reference strains from culture collections.MethodsThe minimal inhibitory concentration (MICs) and minimal bactericidal concentration (MBCs) were determined by using the broth macrodilution method. The kinetics of the inhibitory effects of Mul-1867 against biofilm-growing microorganisms was assessed at time-kill test in vitro against 48-h-old biofilms of Staphylococcus aureus and Escherichia coli. Transmission electron microscopy analyses was conducted to examine cell disruption.ResultsA comparative assessment of the antimicrobial activities of Mul-1867 and chlorhexidine digluconate (CHG), used as a control antimicrobial, indicated that Mul-1867 was significantly more effective as a disinfectant than CHG. Mul-1867 showed potent antimicrobial activities against all the tested bacteria (MIC: 0.03–0.5 μg/mL). Furthermore, MBC/MIC ratio of Mul-1867 for all tested strains was less than or equal to 4. Time-kill studies showed that treatment with Mul-1867 (0.05–2 %) reduced bacterial numbers by 2.8–4.8 log10 colony forming units (CFU)/mL within 15–60 s. Bactericidal activity of Mul-1867 was confirmed by morphological changes revealed by TEM suggested that the killing of bacteria was the result of membrane disruption.ConclusionOverall, these data indicated that Mul-1867 may be a promising antimicrobial for the treatment and prevention of human infections.

Highlights

The antimicrobial activity of Mul-1867, a novel synthetic compound, was tested against 18 bacterial strains, including clinical isolates and reference strains from culture collections
Antibiotic and antiseptic resistance have partly emerged by the prevalence of bacteria in the form of biofilms, which enable microorganisms to survive antibiotic concentrations that are 1000 times higher than the minimal inhibitory concentration (MIC) [4,5,6]
Bacterial strains Bacillus cereus VT-289, Enterococcus faecalis VT-72, E. faecalis VT-693, Escherischia coli VT-1402, K.pneumoniae VT-1367, Neisseria subflava VT-455, Pseudomonas aeruginosa VT-177, Salmonella enterica VT-191, S. аureus MSSA VT 961, S. aureus MRSA VT −234, Streptococcus epidermidis VT-432, S. mitis VT-842, and S. pyogenes VT 59, S. epidermidis VT 908 were obtained from a private collection

Summary

Introduction

The antimicrobial activity of Mul-1867, a novel synthetic compound, was tested against 18 bacterial strains, including clinical isolates and reference strains from culture collections. Infectious disease treatments are associated with challenges including increasing antimicrobial resistance, drug cytotoxicity, and limited drug spectrum and these difficulties have instigated novel antimicrobial drug development [1,2,3]. Topical antimicrobial agents are routinely used in various branches of medicine such as dentistry, otolaryngology, surgery and gynecology [12,13,14,15,16]. They are used to reduce nosocomial infections and are useful in preventing infections in intensive care unit patients [17,18,19]. The above-mentioned properties of CHG have enabled its incorporation in numerous pharmaceutical and medical devices [24, 25]

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