In Vitro Antimicrobial Activity and Fungitoxicity of Syringic Acid, Caffeic Acid and 4-hydroxybenzoic Acid against Ganoderma Boninense

Khim Phin Chong,Markus Atong,Stephen Rossall

doi:10.5539/jas.v1n2p15

Abstract

This paper discusses the in vitro antimicrobial activity and fungitoxicity of syringic acid, caffeic acid and4-hydroxybenzoic acid which is found in oil palm root. Experiments were observed for fourteen days, repeated at leastthree times and data were recorded daily. The antimicrobial activities and fungitoxicity of the phenolics againstGanoderma boninense were expressed in inhibition of radial growth of G. boninense on PDA ameliorated with the threedifferent phenolics with a range concentration of 0.5-2.5 mg/ml. Syringic acid was found to be very fungitoxic to G.boninense even at concentration of 0.5 mg/ml, the lowest concentration tested in this experiment. When theconcentration is increase to 1.0mg/ml of syringic acid, the pathogen is inhibited. Caffeic acid and 4-hydroxybenzoicacid were having inhibitory effect with the highest concentration tested; 2.5mg/ml strongly inhibited the growth of G.boninense in comparison to the control.

Highlights

(Nazeeb et al, 2000)
The antimicrobial activities and fungitoxicity of the phenolics against Ganoderma boninense were expressed in inhibition of radial growth of G. boninense on Potato Dextrose Agar (PDA) ameliorated with the three different phenolics with a range concentration of 0.5-2.5 mg/ml
A series of 0, 0.5, 1.0, 1.5, 2.0 and 2.5 mg/ml of syringic acid, caffeic acid and 4-hydroxybenzoic acid from Sigma® were ameliorated into the PDA, which the phenolics were first dissolved in Acetone: Water (50:50; v/v)

Summary

Introduction

(Nazeeb et al, 2000). Heavily infected field yielded 26% less at 11 years after planting, and 46% less at 15 years by which time incidence was 67% (Gurmit, 1991). Determination of total phenolic content in G. boninense infected and healthy oil palm roots showed susceptible palm roots at week four had low phenolic content, whereas week one had high phenolic content. Determination of total phenolic content in infected palm seedlings root (DX P) showed low phenolic content compared to the non infected palm seedlings root. This indicate phenolic compounds are involved in oil palm resistance against Ganoderma (Mohamad Arif et al, 2007).To identify the possibility of oil palm resistance against G. boninense in certain circumstances need further investigation. In a collaborative experiment to this research, we have found syringic acid, caffeic acid and 4-hydroxybenzoic acid present in oil palm roots in natural condition or after elicitation. We present the works on in vitro effect of syringic acid, caffeic acid and 4-hydroxybenzoic acid to G. boninense

Methods

Results

Conclusion