Abstract
The different parts of Physalis philadelphica were evaluated for the antifungal, anti-oxidant and HPLC assay. The methanol extract of leaf showed inhibitory activity against Pencillium digitatum (58% ZI), Aspergillus flavus (95% ZI) and Rhizopus oryzae (96% ZI) at a concentration of 400 ppm. Strong anti-oxidant activities were found in different parts of the plant. The effects of different extraction solvents on the anti-oxidant activity and antifungal activity of the medicinal extracts were observed. The methanol extract from the leaf, stem, fruit and water fractions from the calyx exhibited strong anti-oxidant activity of 94, 89, 88 and 93% respectively. The ethyl acetate extract was found as a best solvent for the recovery of gallic acid in HPLC assay. No correlation was observed between the gallic acid and anti-oxidant capacity of the extracts.
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Highlights
Fungal infection of grains is common in agriculture depending countries and causes significant loss of foodstuffs by retarding its nutritive value and make them unfit for human consumption (Devi et al, 2001; Marin et al, 1999)
Potato dextrose agar (70139 Sigma-Aldrich), potato dextrose broth (P6685 Sigma-Aldrich), dimethyl sulfoxide (DMSO) (1211006 Sigma-Aldrich), gallic acid (91215 Sigma-Aldrich), 2,2-diphenyl-1-picrylhydrazyl (D9132 – DPPH) and fluconazole were purchased from the local supplier
The different solvents extracted samples of leaves were active against A. flavus except for water extract (Figure 2B)
Summary
Fungal infection of grains is common in agriculture depending countries and causes significant loss of foodstuffs by retarding its nutritive value and make them unfit for human consumption (Devi et al, 2001; Marin et al, 1999). A large portion of the peoples in the developing countries is suffered from the health problems associated with mycotoxin contamination of grain and cereal (Majumder et al, 1997). Due to the toxic effect of synthetic fungicide, it cannot be applied directly to grains (Dukic et al, 2004). Secondary metabolites of plants with pesticidal activity are one of the best alternatives to synthetic chemicals, with least toxic effect and ecofriendly (Varma and Dubey, 1999). This led to researchers to screen out the natural flora for the presence of antifungal bioactive compounds
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