In vitro antifungal activity of lime peels extract (Citrus aurantifolia (Cristm.) Swingle.) against Fusarium oxysporum Schlecht. em. Snyd. & Hans.

Abstract

Fusarium wilt in plants is caused by Fusarium oxysporum. Fusarium wilt disease prevention using synthetic fungicides cause various problems in the environment and human health, it is necessary to change to natural fungicides like lime peels. This study was conducted to determine the percentage of antifungal activity at the optimum concentration of ethanol extract of lime peel (Citrus aurantifolia) to inhibit the growth of Fusarium oxysporum. Lime peel extract was obtained from the maceration process using 96% ethanol solvent. Antifungal activity test uses a food poisoning method with mycelium diameter parameters that grow on the media. The concentration of ethanol extract of lime peels tested was 0%, 15%, 30%, 45%, and 60%, and the positive control used was synthetic antracol fungicide 0.3%. The results showed the optimum concentration of ethanol extract of lime peel to inhibit the growth of Fusarium oxysporum was a concentration of 15% with a percentage of antifungal activity is 56.34%. This is because the concentration is the lowest concentration that can inhibit the growth of Fusarium oxysporum with a strong level of activity.