In vitro antifungal action of different substances over microwaved-cured acrylic resins

Henrique Montagner,Brenda Paula Figueiredo De Almeida Gomes,Paulo Edelvar Correa Peres,Katia Olmedo Braun,Francisco Montagner

doi:10.1590/s1678-77572009000500015

Henrique Montagner, Brenda Paula Figueiredo De Almeida Gomes + Show 3 more

Open Access

https://doi.org/10.1590/s1678-77572009000500015

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Abstract

Objective:The presence of Candida albicans on the surfaces of denture-base acrylic resins is strongly related to the development of oral stomatitis. This study evaluated the antifungal action of different agents over microwave-cured acrylic resin without polishing specimens previously contaminated with Candida albicans.Material and Methods:Sixty specimens were immersed in BHI broth previously inoculated with the yeast and stored for 3 h at 37°C. They were divided into 5 experimental groups (n=10): G1: 2% chlorhexidine solution (10 min); G2: 0.5% sodium hypochlorite (10 min); G3: modified sodium hypochlorite (10 min); G4: effervescent agent (5 min); G5: hydrogen peroxide 10v (30 min). The specimens of the control group 1 (C1) were not disinfected. Ten additional specimens of the control group 2 (C2) were not infected with the yeast, aiming to check the asepsis during the experiment. The disinfection agents were neutralized and the acrylic resin specimens were immersed in BHI Broth for 24 h. Culture media turbidity was evaluated spectrophotometrically according to the transmittance degree, i.e. the higher the transmittance the stronger the antimicrobial action. Statistical analysis was performed (Kruskal-Wallis Test, p<0.05).Results:The results, represented by the medians, were: G1 = 40; G2 = 100; G3 = 100; G4 = 90; G5 = 100; C1 = 40; C2 = 100.Conclusions:This in vitro study suggested that sodium hypochlorite-based substances and hydrogen peroxide are more efficient disinfectants against C. albicans than 2% chlorhexidine solution and the effervescent agent.

Highlights

The presence of bacterial plaque and fungi are the main etiological factors of denture-induced stomatitis[19]
The results of the spectrophotometer measurements were represented by medians and minimum and maximum values, as follows: G1 – 2% chlorhexidine solution = 40; G2 – 0.5% sodium hypochlorite = 100; G3 – Modified sodium hypochlorite = 100; G4 – effervescent agent = 90; G5 – 10 volumes hydrogen peroxide = 100; control group 1 (C1) – specimens without disinfecting agents = 40; control group 2 (C2) – specimens without contamination = 100
Chlorhexidine solution and the non-disinfected specimens did not inhibit the growth of the target microorganism, as observed by the microbial growth on the agar plates

Summary

Introduction

The presence of bacterial plaque and fungi are the main etiological factors of denture-induced stomatitis[19]. According to Ramage20, 11% to 67% of complete denture users present candidiasis. Denture-induced stomatitis is characterized by the alteration of tissues under dentures, such as the presence of bright red colored areas and wrinkled surfaces[12]. Indication of denture cleansing is of paramount importance[23]. The inner surface of dentures acts as a C. albicans reservoir, which is responsible for the beginning, progression and maintenance of the infection. Measures for controlling the colonization by this fungus may be adopted by the users of dentures[12,22]. A routine of cleaning may be instituted to prevent and remove the accumulation of microorganisms and to remove mucine, food remains, calculus and stains

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