In vitro antidiabetic effect of Leptedenia hastata leaves fractions

Abstract

Leptadenia hastata (Pers.) Decne is often used traditionally for hypertension, catarrh, skin diseases, wound-healing and in the management of diabetes mellitus. Hydromethanolic leaves extracts of Leptadenia hastata were analysed for their phytochemical contents while its fractions were subjected to in vitro antidiabetic activity using non-enzymatic glycosylation of hemoglobin, increase in glucose uptake by yeast cell and α-amylase inhibition assays respectively. Phytochemical screening of L. hastata leaf indicated the presence of tannins, flavonoids, alkaloids and saponins. The results indicated significant inhibition of glycosylation of haemoglobin, glucose uptake by yeast cells and αamylase inhibition was in a dose-dependent manner respectively. The present findings suggest that Leptadenia hastata fractions exhibited antidiabetic effects thus validating the traditional claim of this plant. Citation: Ukwuani AN and Igbokwu MO (2015). In vitro antidiabetic effect of Leptadenia hastata leaves fractions. Biosciences Research in Today’s World 1(1): 40-46. Received May 29, 2015; Accepted July 2, 2015; Published July 19, 2015. Copyright: © 2015 Ukwuani and Igbokwu. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. BRTW is the official journal publication of BRSF. Competing Interests: The authors have declared that no competing interests exist. * E-mail: pinknnenna@yahoo.com This research work was presented at the Biosciences Research Support Foundation (BRSF)’s 1st International Conference on Biosciences Research (ICBR), Awka, Nigeria, 25-27 May 2015.