Abstract
Active compounds of Swietenia mahagoni seed have power as anti diabetic that can be used in diabetes mellitus treatment. S. mahagoni seeds were extracted using methanol-maceration, followed by partitioning with petroleum ether (PE), chloroform (CL), ethyl acetate (EA), and dia-ion resin (DR). Column chromatography was used to further separate the dia-ion Resin fraction in order to get isolate purer compounds. One of the strategies is maintaining postprandial glucose level through inhibition of α-amylase and α-glucosidase. So that pre-prandial and post-prandial glucose levels can be controlled properly. The aim of this study was to determine in vitro inhibitory activity of Swietenia mahagoni seed by α-glucosidase, α-amylase. Inhibitory activity was measured using spectrophotometric method, α-amylase activity was measured at λ = 540 nm, α-glucosidase activity measured at λ = 405 nm. DRF inhibit both α-glucosidase and α-amylase better than acarbose as a positive control. This study showed that some extracts and some isolated compounds have inhibition activity of α-glucosidase and α-amylase. According to the findings, DR fraction (IC-50 = 328.22μg/mL) had a higher level of alpha-glucosidase inhibitory activity than acarbose (IC-50 = 336.95μg/mL). The six DR fractions compounds were separated using column chromatography. The DR fraction (40.62%), (50.39%), showed the highest inhibition activity.
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