Abstract
Objective: This present study aimed to evaluate the anticoagulant activity and antioxidant properties of Prasaplai recipe (PPR), a Thai traditionalmedicine, and its major ingredient, Zingiber cassumunar (ZC) Roxb. extracts, seeking new therapeutic purposes for the recipe.Methods: Aqueous extracts of PPR and ZC Roxb. were prepared by hot water decoction technique. The anticoagulant activity of the extracts wasevaluated by prothrombin time (PT) and activated partial thromboplastin time (APTT) tests. In addition to anticoagulant activity, total phenolcontent and antioxidant activity were investigated. Total phenol content was determined using the Folin–Ciocalteu assay. The antioxidant activity wasestimated by DPPH radical scavenging activity and ferric reducing antioxidant power assay.Results: The APTT of plasma samples mixed with the PPR and ZC Roxb. extracts was significantly prolonged (p<0.05) at the concentration of1.0 mg/ml and above comparing to the control (normal saline solution) but was no significantly different for the PT. These results suggested thatPPR and ZC Roxb. extracts showed anticoagulant activity affecting the function of coagulation factor in the intrinsic pathway. All aqueous extractspossessed considerable antioxidant activity and were rich in total polyphenol.Conclusion: This finding indicates that the aqueous extracts possess significant anticoagulant and antioxidant activities, thus showing the potentialPPR and ZC Roxb. as a new source of bioactive compounds for therapeutic purposes, with particular emphasis on the prevention and treatment ofthrombosis.
Highlights
Thai traditional herbal medicines are used for the treatment of various symptoms and diseases [1]
The crude extract of Prasaplai recipe (PPR) was evaluated for anticoagulant activity at the concentrations of 0.5, 1.0, 2.0, and 4.0 mg/m1 while the extract of Zingiber cassumunar (ZC) Roxb. was determined for anticoagulant activity at the selected concentration, 1.0 mg/ml (Table 1)
We have described the in vitro anticoagulant activity of aqueous extracts from PPR and ZC Roxb., which are beneficial in Thai traditional medicine
Summary
Thai traditional herbal medicines are used for the treatment of various symptoms and diseases [1]. Known as Plai, is one of the Thai herbal formulas which is used for restoring body elemental balance [2] It is used for the treatment of menstrual disorders, flow enhancement of the discarded uterine blood after birth, wound healing, muscle and joint pain, skin diseases, inflammation, and abscesses [3]. Is a major component of the Prasaplai recipe, a Thai traditional medicine formula, which has been used to relieve pain from the primary dysmenorrhea, regulates irregular menstrual flow, and driving out the discarded uterine blood after birth [2,4]. 1000mg orally 3 times a day before meals is recommended This method is approved by the National Drug List of Herbal Medicinal Products AD 2008 [4]. The previous in vitro studies reported that Prasaplai recipe (PPR) acts as COX inhibitors which can help diminish pain from the primary dysmenorrheal [6]
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