In-vitro anticandidial efficacy of tick egg wax from Hyalomma marginatum, Rhipicephalus bursa and Dermacentor marginatus

Abstract

Objective: In the previous studies, the antibacterial, antifungal, and antiviral efficacy of the tick egg wax-coating of certain tick species wereexamined and some significant results were obtained. However, related researches and studied tick species are limited. There are hundredsof tick species, and it is well known that the antimicrobial efficacy of the wax is closely related to the species. The aim of this study was toinvestigate the in-vitro anticandidial efficacy of the egg waxes belonging to three tick species, which have not been studied before and havequite different biological and ecological differences.Methods: In the study, the egg waxes of the tick species, Hyalomma marginatum, Rhipicephalus bursa, and Dermacentor marginatus, wereused on Candida albicans ATCC10231, Candida parapsilosis ATCC 22019, and Candida tropicalis ATCC 750. Antimycotic susceptibility test wascarried out in accordance with the Clinical and Laboratory Standards Institute (CLSI) recommendations using the M27-A3 microdilution method.Results: It was determined that the wax of Rhipicephalus bursa has inhibitory effect on Candida tropicalis ATCC 750 in a particular concentration,and no significant effects were observed in other trials.Conclusion: Anticandidial effect obtained from the egg wax of R. bursa can be associated with some distinctive biological characteristics, and itwas concluded that the detailed studies with different tick species might yield significant results for the discovery of new generation antifungals.