In vitro anticancer and antioxidant activity studies on Drosera peltata J.E.Sm.

Abstract

Aim: The present study aimed to assess the in vitro antioxidant and anticancer properties of Drosera peltata J.E.Sm. Methods: Antioxidant capacity of the extracts were tested on hydroxyl, DPPH, super oxide, nitric oxide and ABTS radicals; of ferrous ion chelating ability and by reducing power assay. Ascorbic acid was used as the standard antioxidant for the above all assay methods. Dalton’s Ascitic Lymphoma (DAL) and Ehrlich Ascitic Carcinoma (EAC) were used as cell lines to assess its in vitro anticancer effect. Tryphan blue dye and LDH leakage assays were carried out to know its anticancer potential. Moreover, total flavonoid content was estimated to find out the exact reason for its antioxidant potential. Results: The aqueous extract (AEDP) in comparison with the ethanol extract (EEDP), showed considerable antioxidant and anticancer activities in all the models tested. The minimum IC50 values of 48.87 ± 0.53 mcg/ml was shown by EEDP in metal chelating assay and 28.46 ± 0.7 mcg/ml by AEDP in hydroxyl radical scavenging assay. Higher doses of both extracts (250mcg/ml) showed significant cytotoxic effects on DAL and EAC of LDH leakage assay model. Total flavonoid contents of both extracts were estimated and expressed as mg/g quercetin equivalent (QE), where ethanol extract was rich in flavonoid content than aqueous extract. Conclusion: These study results were concluded that D. peltata exhibited excellent antioxidant activity against free radicals which were generated from different models and showed very good anticancer activity against the two cell lines, this results might be due to the phytoconstituent such as flavonoid present in the plant extracts.