In Vitro Antibacterial Experiment of Fuzheng Jiedu Huayu Decoction Against Multidrug-Resistant Pseudomonas aeruginosa.

Hongri Xu,Mingzhe Wang,Meng Chen,Xingwei Yao,Miao Cheng,Meng Li,Chengxiang Wang,Jie Ma,Honghong Wang,Chang Liu,Guoxing Liu,Lei Li,Yuting Wang,Ying Lin,Shitong Zhao

doi:10.3389/fphar.2019.01682

Abstract

ObjectiveDrawing a growth curve of multidrug-resistant Pseudomonas aeruginosa (MDR-PA) provides a foundation for susceptibility testing. By observing in vitro antibacterial activity and ultrastructure cthanges on MDR-PA of the effective components in the drug-containing serum of rats after the administration of Fuzheng Jiedu Huayu decoction (FJHD), we evaluated the inhibition and direct destruction effect of bacteria by TCM alone or combined with antibiotics.MethodsThe absorbance values of MDR-PA were determined at different detection time points, and a growth curve was drawn. After gavage with FJHD, drug-containing serum was collected from the rats. Using Imipenem/cilastatin sodium as the positive drug control, the in vitro antibacterial potency of FJHD and its drug-containing serum alone or in combination with antibiotics against MDR-PA was observed. The ultrastructural changes of MDR-PA treated by FJHD combined with antibiotics were observed by transmission electron microscopy.ResultsGrowth of the experimental strain manifested a lag phase in the first 1–4 h, an exponential growth phase at 5–20 h, and a plateau phase after 20 h. The best detection time during the susceptibility test was 16–20 h. The minimum inhibitory concentration (MIC) value of the FJHD extract group was 0.2 g/mL. The MIC value of the pure Imipenem/cilastatin sodium group was 16 μg/mL. The MIC values of Imipenem/cilastatin sodium + blank serum, 0.5-, 1-, and 2-fold drug-containing serum groups were all 16 μg/mL. The MIC values of Imipenem/cilastatin sodium + 4- and 8-fold drug-containing serum groups were both 8 μg/mL. By observation under a transmission electron microscope, Imipenem/cilastatin sodium + 0.5-, 1-, and 2-fold drug-containing serum groups showed bacterial structural damage. The degree of bacterial destruction was more obvious and the quantity of damaged bacteria was increased in the Imipenem/cilastatin sodium + 4- and 8-fold drug-containing serum groups.ConclusionDrawing the growth curve of the experimental strain had high application value for ensuring the accuracy of the drug sensitivity test results. TCM combined with antibiotics could enhance the antibacterial and direct destruction effect of bacteria in vitro, thereby inhibiting bacterial resistance to a certain extent.

Highlights

Due to the extensive application of antibacterial drugs worldwide, the problem of bacterial resistance is becoming increasingly serious
Statistical Results of MDR-Pseudomonas aeruginosa (PA) Electron Microscopy According to the photomicrograph of the electron micrograph, Imipenem/cilastatin sodium + 0.5-fold, 1-fold, and 2-fold drugcontaining serum groups showed bacterial structure damage, similar to the simple Imipenem/cilastatin sodium group, and there was no significant difference
In a previous clinical study (Xu et al, 2018), our research group confirmed that Fuzheng Jiedu Huayu decoction (FJHD) full active ingredient extract formula granules significantly improved the effective rate with a course of treatment for more than 2 weeks in the elderly patients with pneumonia

Summary

Introduction

Due to the extensive application of antibacterial drugs worldwide, the problem of bacterial resistance is becoming increasingly serious. Prospective, double-blind, parallel, randomized controlled trial (Xu et al, 2018), we found that Fuzheng Jiedu Huayu decoction (FJHD) full active ingredient extract formula granule, based on the above basic TCM pathogenesis of pneumonia in the elderly, significantly improved the curative effects of patients after a 2-week treatment course, ameliorating expectoration, and promoting the absorption of pneumonia lesions. FJHD full active ingredient extract formula granules showed effectiveness in the treatment of elderly drug-resistant pneumonia in previous clinical research (Xu et al, 2018). To further explore and reveal the mechanism of FJHD full active ingredient extract formula granules for treating pneumonia caused by drug-resistant bacteria infection, the antibiotic Imipenem/cilastatin sodium, which is commonly used in clinical practice and has strong antibacterial effect, was used as the positive drug control. The growth curve of MDR-PA was drawn, and the best detection time point was determined to ensure the accuracy of the drug sensitivity test results

Methods

Results

Conclusion