In Vitro Antibacterial Effect of Four Medicinal Plant Extracts on Brucella Abortus Isolated from Cattle

Abstract

Background: Brucella abortus is the main causative agent of an infectious zoonotic disease known as brucellosis in both human and livestock. The most commonly used vaccine, B. abortus S99 for brucellosis has often given false positive results in mature animals which has brought a high risk for the bacteria vaccine to humans, thus the option for new antibacterial compounds are becoming necessary for efficient brucellosis treatment. This study aimed to investigate the antibacterial activity of extracts of Lepidium sativum, Solenostemma argel, Organum marjoram and Eucalyptus camaldulensis. Methodology: Aqueous, hexane and alcohol extracts of Lepidium sativum, Solenostemma argel, Origanum marjoram and Eucalyptus camaldulensis were used to determine the antibacterial activity using well diffusion test; inoculation broth method and disc diffusion method at different concentrations, using Streptomycin as a positive control. Results: The results obtained showed different inhibition zones with different herbs, Lepidium sativum (46mm/20%/ hexane extract /well test), Solenostemma argel (36mm /20%/hexane extract /disc test) and Marjoram origanum (20 mm /20%/ethanol , hexane extracts /well test). No inhibition was observed in case of Eucalyptus camaldulensis extracts. Streptomycin reached inhibition (56mm) in Lepidium sativum hexane extract dish;(56mm) in the Solenostemma argel ethanol extract dish and extract (52mm) in the Marjoram origanum ethanol extract.