In vitro Antibacterial Activity Of Ethanolic Extract of ‘Calliandra Haematocephala’ against Selected Bacterial Strains

Glory Josephine I,Muthiah N S,Punnagai K

doi:10.13005/bpj/1230

Abstract

Introduction Herbals have been used as therapeutic agents for longer period in our Traditional medical system. Considering the emergence of the multidrug resistance, cost and adverse effects of the antibiotics these natural sources provide nice opportunities for new drug leads. Also the therapeut

Highlights

Herbals have been used as therapeutic agents for longer period in our Traditional medical system
Bacterial susceptibility testing In vitro Antibacterial activity of extract was determined against three specimens of gram positive bacteria (Staphylococcus aureus, Bacillus subtilis and Micrococcus leteus ) and seven gram negative bacteria specimens (Salmonella typhi, Serratia marcescens, Escherichia coli, Shigella flexneri, Vibro parahymolyticus, Pseudomonas aeroginousa and Enterococcus,) by agar disc diffusion method on Muller Hinton agar (MHA) medium
Results showed the antibacterial activity of Calliandra haematocephala leaves on all strains

Summary

INTRODUCTION

Herbals have been used as therapeutic agents for longer period in our Traditional medical system. It is reported that betulinic acid in calliandra is responsible for its antitumor, anti –HIV [5] and anti rotaviral activity [6] It is used as antibacterial agent [7] and studies showed about its antibacterial activity of bark and flowers. One study showed the antibacterial activity of silver nanoparticles synthesized using the leaf extract of Calliandra haematocephala against E. coli. In another study in vitro investigation of extract obtained from flowers of the shrub Calliandra haematocephala showed greater activity against gram positive bacteria Staphylococcus aureus [9] Disc diffusion method is one of the reliable, most commonly used in vitro screening methods to evaluate the antibacterial activity of various drugs and phytochemicals . Since the above findings suggested for strong antibacterial activity, we designed this study to prove the antibacterial activity of the ethanolic extract of the leaves of Calliandra haematocephala against selected gram positive and gram negative microorganisms using the in vitro standard disc diffusion method

MATERIALS AND METHODS

RESULTS

DISCUSSION