Abstract

Biosynthesis of silver nanoparticles using Planomicrobium sp. and to explore the antibacterial activity against food borne pathogenic bacteria Bacillus subtilis, (3053) Klebsiella planticola (2727) Klebsiella pneumoniae (MAA) Serratia nematodiphila (CAA) and Escherichia coli. In the current studies, 1 mM of silver nitrate was added into 100 mL of Planomicrobium sp. culture supernatant. The bioreduction of pure AgNO3 was characterized by UV-visible spectroscopy, X-ray diffraction analysis (XRD), scanning electron microscopy (SEM), energy-dispersive analysis (EDS), transmission electron microscopy (TEM), and Fourier transform infrared (FT-IR) analysis. The formation of silver nanoparticles was confirmed by the presence of an absorption peak at 400 nm using UV-visible spectrophotometry. The morphology and size of the silver nanoparticles was monitored by TEM and SEM. Crystal structure was obtained by carrying out X-ray diffraction studies and it showed face centered cubic (FCC) structure. The bactericidal effect of silver nanoparticles was compared based on diameter of inhibition zone in well method. Bacterial sensitivity to nanoparticles a key factor in manufacture the suitable for long life application in food packaging and food safety. Food safety is a worldwide health goal and the food borne diseases get a main disaster on health. Therefore, controlling of bacterial pathogens in food is credit of harms associated to health and safety.

Highlights

  • Nanotechnology in the food manufacturing can get a number of forms

  • A serious problem in food packaging is that of migration and permeability [2, 3]: no stuff is totally resistant to water vapor, atmosphere level, or moisture controlled within the food being packaged

  • Though some techniques are capable of decontaminating orange juice, recently nanotechnology introduced in the food packaging industry can potentially provide solutions to food packaging challenges

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Summary

Introduction

Nanotechnology in the food manufacturing can get a number of forms. It takes in the use of nanotechnology in food packaging materials. Packaging can give to the control of bacterial growth in food stuffs [1], which can the way to in the container of pathogenic bacteria, illness and disease. Though some techniques are capable of decontaminating orange juice, recently nanotechnology introduced in the food packaging industry can potentially provide solutions to food packaging challenges. The metal nanoparticles are broadly used in several biomedical and bioelectrochemical applications due to their extraordinary electrocatalytic activity. It is well-known to make silver nanoparticle is an effective antibacterial agent and possesses a strong antimicrobial activity against bacteria, viruses and fungi; even the mechanism and way of action are still not well known [14]

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