Abstract
Acetylcholinesterase is a key enzyme that terminates nerve impulses by catalyzing the hydrolysis of neurotransmitter, acetylcholine, in the nervous system in various organisms. Irreversible inhibitors have been developed as insecticides such as organophosphates and carbamates. Aqueous crude fruit sap extract of S. incanum was evaluated for acetylcholinesterase activity in green peach aphids using Ellman’s method and is found active in inhibiting the ACHe of green peach aphids. All tested concentrations of crude fruits sap extract of S. incanum possessed the ability to inhibit acetylcholinesterase of green peach aphids at dose dependent manner and IC50 of 49.9 was calculated. This study suggest that aqueous crude fruit sap extract of S. incanum inhibit the acetylcholinesterase of the green peach aphids and it is possible the extract has compounds that increase both the level and duration of the neurotransmitter action.
Highlights
Acetylcholinesterase (AChE) is an enzyme that terminates nerve impulses by catalyzing the hydrolysis of neurotransmitter, acetylcholine, in the nervous system in various organisms [1]
Assay reagents: The assay reagents used was acteylcholinesterase assay kit ab138871 which contains acetylcholinesterase standard, assay buffer, 5, 5′-dithio-bis (2-nitrobenzoic acid) (DTNB) and acetylthiocholine were purchased from Abcam (UK), bovine serum albumin (BSA), Commassie brilliant blue and Phosphoric acid (Sigma aldrich, USA), Triton X-100 (Lobal Chemie, India) and EDTA (Duchefa Haarlem, Netherlands)
Acetylcholinesterase affects the cholinergic system because it regulates the level of acetylcholine and terminates nerve impulses by catalyzing the hydrolysis of acetylcholine
Summary
Acetylcholinesterase (AChE) is an enzyme that terminates nerve impulses by catalyzing the hydrolysis of neurotransmitter, acetylcholine, in the nervous system in various organisms [1]. Solanum incanum is a perennial, wild shrub like herb that belongs to family Solanaceae, which grows in many regions of Africa, Middle East and Far East Asia. It is an erect or spreading perennial shrub with leaves and stem occasionally having small prickles. The fruits are small berries of 2-3 cm in diameter and yellowish orange or brown in color when ripe [3] It is common as a weed around houses, in overgrazed grass land and on road sides. It found at forest edges and in bush land and grasslands, from sea-level up to 2500 m altitude [4]
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