In Vitro Anti-Wrinkle and Skin-Moisturizing Effects of Evening Primrose (Oenothera biennis) Sprout and Identification of Its Active Components

Tae Heon Kim,Woo Jung Kim,Soon Yeong Park,Hoon Kim,Dae Kyun Chung

doi:10.3390/pr9010145

Tae Heon Kim, Woo Jung Kim + Show 3 more

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https://doi.org/10.3390/pr9010145

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Abstract

The present study aimed to investigate the effect of Oenothera biennis sprout extract (OBS-E) on skin-function improvement in an in vitro system and to identify its pharmaceutically active components. OBS-E showed antioxidant ability in radical scavenging and reducing power assays, significantly inhibited matrix metalloproteinases-1 and -2, and increased the production of type I collagen, indicating its anti-wrinkle activity. Furthermore, OBS-E significantly increased the level of hyaluronic acid (HA) and the expression of moisturizing genes, such as hyaluronic acid synthase 2 (HAS2) and aquaporin 3 (AQP3), indicating it is effective in enhancing skin hydration. High-performance liquid chromatography (HPLC) and mass spectrometry (MS) analyses showed that OBS-E contained high levels of polyphenolic acids, such as gallic acid and ellagic acid, in addition to flavonoid glycosides, such as luteolin 7-glucuronide and quercetin 3-glucuronide. Our results suggest that these major phytochemicals are likely to play crucial roles in the expression of antioxidant, anti-wrinkle, and moisturizing activities of OBS-E.

Highlights

The skin is the largest and one of the most important organs of the human body, providing a barrier that prevents the entry of hazardous substances and pathogens inside the body, while regulating temperature and controlling under- or over-hydration, maintaining essential chemicals and nutrients, and preventing the harmful effects of sunlight [1]
We aimed to investigate the potential use of Oenothera biennis sprout (OBS)-E as a new nutraceutical ingredient for the enhancement of skin health
Our results showed that Oenothera biennis Sprout Extract (OBS-E) has the possibility of utilization as an antioxidant agent

Summary

Introduction

The skin is the largest and one of the most important organs of the human body, providing a barrier that prevents the entry of hazardous substances and pathogens inside the body, while regulating temperature and controlling under- or over-hydration, maintaining essential chemicals and nutrients, and preventing the harmful effects of sunlight [1]. Apart from the maintenance of a healthy skin by the application of cosmetics (beautification from the outside), recent skincare interests include those directed toward achieving beautification from the inside, achieved by the oral administration of functional and medicinal substances [2]. 21th century, many researchers and cosmeceutical developers have been attempting to find novel substances that have positive influences on human skin health when consumed orally. Medicinal plants are generally recognized as safer and having fewer adverse effects. They are becoming widely used in diverse fields, such as pharmacology, cosmetics, perfumery, nutraceuticals, beverages, and dyeing industries [4,5]. The genus Oenothera, known as evening primrose, contains approximately 145 species of herbaceous flowering plant [6]

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