In vitro Anti-Proliferative Evaluation of Tridax procumben Plant on Colon Cancer Cell Lines

Abstract

Every year it is estimated that 9.6 million deaths occur due to cancer disease according to the current census. Therefore, development for novel, safe and efficacious drug for treating cancer is needed. Medical plants are used for the treatment of various types of cancer since ancient times. In the present study, one attempt has been made to explore the medicinal properties of Tridax procumbens L. To prepare plant extract of Tridax procumbens using maceration method and to evaluate in vitro anti-proliferative activity on colon cancer cell line by MTT assay of the plant extract, the Tridax procumbens plant was used involving anti-proliferative potential as hydroalcoholic extract using ethanol and water (70:30v/v). The preliminary phytochemical analysis resulted in the presence of phyto-constituents in hydroalcoholic plant extract and cytotoxic potential was carried out on colon cancer cells (HT-29) using cell viability by MTT assay and plant extract showed significant anti-proliferative activity on colon cancer cells with IC50 value (74.10 μg/ml) as compared with normal epithelial cells (IEC-6) which showed the non-toxic effects. Hence it can be stated that Tridax p has excellent anti-proliferative activity and plant extract can be used in making of herbal based anticancer drugs which can be less expensive and available to all.