In vitro Anti-Inflammatory Egg Albumin Denaturation Assay: An Enhanced Approach

Abstract

As a physical reaction to injury, infection, or destruction, inflammation is characterized by heat, redness, discomfort, swelling, and abnormal physiological processes. The inflammatory process is a complicated series of relationships among soluble substances and cellular elements that frequently include connective tissue damage. An uncontrolled inflammatory response is the primary cause of a wide range of disorders, including allergies, cardiovascular dysfunctions, metabolic syndrome, cancer, and autoimmune diseases, placing a significant financial burden on individuals and, in turn, on society. Steroids, nonsteroid anti-inflammatory drugs, and immunosuppressants are some examples of common medications for controlling and suppressing inflammatory crises; but they have several side effects, particularly stomach irritation that can cause gastric ulcers. Using natural goods has had a significant positive impact on the development of modern medicine. On such background, plants have historically been an excellent source of medication, and there are several records on the use of herbs in traditional medicine to treat a variety of inflammatory illnesses. However, there has been no scientific attempt to verify these spontaneous uses reported in the literature. Therefore, to conduct more studies and gather more data, scientists need to develop low-cost, straightforward in vitro methodologies to assess the efficacy of natural anti-inflammatory compounds. With this context in mind, this methodology focuses on in vitro anti-inflammatory methodology that can be used to evaluate natural anti-inflammatory substances. They are cost-effective, easy to operate, and dependable with minor alterations.