Abstract
Aim: To define the putative anti -inflammatory and cytotoxic effects of ibidI AE, a new phytotherapeutic formulation composed of three ex tracts:Punica granatumL, pericarpum; Boswellia serrataRoxb., resina;Curcuma longa L,. rhizome,using Caco-2 cells, an in vitro model of human intestinal epithelium. Methodology : cytotoxicityand capacity of ibidIAE to induce cell proliferation were assessed respectively by2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5Carboxanilide(XTT) assay andnucleotide 5bromo-2 � -deoxyuridine(BrdU) incorporation. Cell migration was evaluated by scratch wound assay. COX -2, IL-6, IL-8 and MCP -1 proteinlevels were measured in the supernatant of cells stimulated with or without TNF alfa or IL-1 beta in presence or in absence of ibidIAE using ELISA assays. Finally, the influence of ibidIAE on the integrity, paracellular permeability, and viability of Caco -2 cell
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