In vitro Anti-cancer, Anti-inflammatory and Anti-arthritic activity of Ethanolic extract of Ochna obtusata leaves

Abstract

Objective: The objective behind this study was to expose the prospective of ethanolic extract of Ochna obtusata (EEOO) for its in vitro anti-cancer anti-inflammatory and anti-arthritic activities. Methods: The fresh leaves were used to prepare the extracts using petroleum ether, chloroform, ethanol, methanol and water in increasing order of the polarity of solvents. The EEOO was reported to have the presence of a few major phytoconstituents such as, Flavonoids, Phenolics, Glycosides, and Reducing Sugars which was confirmed by preliminary phytochemical screening. The in vitro MTT assay method has been performed with EEOO to evaluate the anti-cancer activity. Similarly, the HRBC Membrane Stabilization assay, Protein denaturation assay, and Protease inhibition assay methods were performed to evaluate the anti-inflammatory and anti-arthritic activity of EEOO. Results and Discussion: From the study, it was confirmed that the various phytoconstituents such as Flavonoids, Phenolics, and Terpenoids were responsible for the anti-cancer activity. Similarly, the prevention of RBC Membrane from its lysis, decrease in albumin denaturation, and protease inhibition was observed which significantly confirmed the anti-inflammatory and anti-arthritic activity of EEOO. Conclusion: It was finally concluded that the phytoconstituents present in the extract were responsible for its anti-cancer, anti-inflammatory, and anti-arthritic activities.