IN VITRO ANTHELMINTIC AND ANTI-AMYLASE PROPERTIES OF GARCINIA PEDUNCULATA ROXB. ETHANOLIC EXTRACT

Pinky Sarmah,Jyotchna Gogoi,Nako Kobing,Ananta Madhab Dutta

doi:10.22159/ijpps.2017v9i1.15395

Pinky Sarmah, Jyotchna Gogoi + Show 2 more

Open Access

https://doi.org/10.22159/ijpps.2017v9i1.15395

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Abstract

Objective: The present study aims to investigate in vitro anthelmintic and anti-amylase properties of the ethanolic fruit extract of Garcinia pedunculata.Methods: For the study, mature fruits of G. pedunculata were collected from local markets during April-May. Fleshy pericarps of fruits were chopped into small pieces, dried and extracted by using a Soxhlet apparatus. Ethanol extract of G. pedunculata was used for evaluation of in vitro anthelmintic and anti-amylase activities. In vitro anthelmintic activity was evaluated in animal models, Pheretima posthuma, an earthworm species. In vitro anti-amylase activity was evaluated by using zymographic, achromic point analysis (Starch-Iodine method) and spectrophotometric method [Di Nitro Salicylic acid (DNS)–Maltose method].Results: Ethanolic extract of G. pedunculata showed anthelmintic activity at a concentration of 75 mg/ml, paralysis and death timing was reported at 0.62±0.26 min and 1.42±0.07 min, respectively. The reference standard (Albendazole) showed paralysis time: 2.13±0.28 min and death time: 5.12±0.29 min. In the anti-amylase study, a zymographic density analysis of G. pedunculata showed significant variation in band intensity as compared to Starch–Iodine achromic point analysis and DNS–Maltose method. A concentration of 1.5 mg/ml of extract showed inhibition of amylase: 67.65±1.53 % as compared to other concentrations and control sets.Conclusion: It could be concluded that ethanolic extract of G. pedunculatahas biological properties which could be utilised in medicine by characterising its bioactive components.

Highlights

In North East India, 9 species of the genus Garcinia (Thekera tenga) have been reported in 1934 which are mainly used in food by different communities [1, 2]
The genus belongs to the family Clusiaceae, the members of this genus are mainly evergreen trees or shrubs with greenish gum resins. They are used in many health problems by different communities like, Kau (G. cowa Roxb.) and Kuji (G. morella Desr.) thekera tenga are used in the treatment of dysentery by Assamese and Bodo people [3]
In the present investigation, G. pedunculata was extracted with ethanol to evaluate in vitro anti-amylase activity

Summary

Introduction

In North East India, 9 species of the genus Garcinia (Thekera tenga) have been reported in 1934 which are mainly used in food by different communities [1, 2]. The enzyme solution was prepared by mixing 1 ml saliva in 19 ml of distilled water. The test tubes were labelled as a zero min control (Blank), plant extract treated and positive control (untreated amylase). Other test tubes contain 1 ml of the extracts with a concentration of 0.5, 1, 1.5 and 2 mg/ml.

Results

Conclusion