In vitro and in vivo study of neutrophil functions after acute methanol intoxication in albino rats

Abstract

Toxicity from methanol (MeOH), a potentially significant problem due to occupational, accidental, or intentional, as well as daily ingestion of small amounts of the agent, only receives considerable attention after severe signs of intoxication have set in or death is imminent. While accidental and intentional exposures usually involve very high doses, the occupational and ingestion forms more often reflect small daily intakes. Still, even at the low levels, little is known about the potential immunotoxic implications (and less so in regard to mechanisms) from these ongoing exposures. This study has been attempted to focus the effects of acute methanol exposure on blood neutrophil functions in both in vitro and in vivo conditions at 30 and 60 min time intervals. Innate immunity of the host defense relies mostly on neutrophils. The blood MeOH levels were determined upon exposing the experimental animals to ¼ of LD50 MeOH. No significant change in the blood methanol level was observed at 30 and 60 min of methanol exposure. The in vitro results showed a decrease in the neutrophil functions of adherence and phagocytic index without affecting the avidity index, followed by an increased nitroblue tetrazolium (NBT) reduction. The in vivo exposures of MeOH showed a decline in neutrophil adherence. However, the other neutrophil functions were found to be significantly increased, which indicates that in vivo results are different from that of the in vitro studies. The present study concludes that MeOH exposure can reconstitute the innate immune status by altering the neutrophil functions. Moreover, the in vivo effects are much pronounced compared to the in vitro effects, which may be due to the formation of the metabolites under in vivo conditions.