In vitro and in vivo investigation of chrysin chelated copper complex as biocompatible materials for bone tissue engineering applications.

Abstract

Flavonoid metal complexes have interesting properties and are widely explored for bone regeneration owing to their potent biological activity. In the present study, we investigated the biocompatibility and osteogenic properties of the Copper(II)-chrysin complex (C/Cu). The biocompatibility of C/Cu was assessed in vitro with human osteoblastic cells and in vivo using chick embryo and zebrafish models. The C/Cu complex was found to be cytofriendly with good biocompatibility. The osteogenic property of C/Cu was studied at cellular and molecular levels. C/Cu promoted mineralization in osteoblastic cultures by increasing ALP activity. At the molecular level, C/Cu significantly promoted the mRNA levels of osteoblast differentiation marker genes such as runt-related transcription factor 2 (Runx2), Type 1 collagen and ALP. In addition to this, secretory proteins, osteonectin (ON) and osteocalcin (OC) levels were also stimulated. We have also identified that C/Cu exhibited enhanced osteogenic properties and antibacterial activity compared with Chrysin. Thus, C/Cu can be used as an osteogenic agent in bone tissue engineering.