Abstract
To investigate whether AKR spontaneous leukaemogenesis is associated with a reduction in functional activity of T lymphocytes, the PHA response of AKR blood cells at different ages up to and including the preleukaemic period was studied. No significant differences were observed among young, adult and preleukaemic donors. In addition, the in vitro and in vivo AKR lymphocyte functions were compared with those of CBA lymphocytes by means of their response to stimulation with T and B lymphocyte selective mitogens (PHA, Con A and LSP respectively), and their response to immunization with thymus dependent (SRBC) or independent (LPS) antigens. We observed in vitro that while the B lymphocytes responded normally to mitogen, an intrinsic hyporeactivity to mitogens characterizes the T lymphocytes. Moreover, AKR mice exhibited a reduced in vivo response to both thymus dependent and independent antigens.
Highlights
We observed in vitro that while the B lymphocytes responded normally to mitogen, an intrinsic hyporeactivity to mitogens characterizes the T lymphocytes
IN 1960, Old and his co-workers reported that mice infected with Friend murine leukaemia virus (F-MuLV) displayed a diminished antibody producing capacity towards sheep red blood cells (SRBC)
Since the leukaemias which develop spontaneously in adult AKR mice originate in the thymus (Metcalf, 1966), it is likely that the thymus derived (T) lymphocytes represent the actual targets for virus induced neoplastic conversion
Summary
Mice.-Inbred AKR, CBA and CBAT6T6 (hereafter called T6) mice from our colony were used throughout. No FCS was added to the medium when cells were used for haemolytic plaque forming cell assay. Blood lymphocyte cultures.-(a) Karyotype analysis assay (Doenhoff et al, 1970): Mixed cultures of AKR and T6 cells contained various proportions of each cell type; the final concentration in all cultures was 2 x 106 cells/ml medium. Antigens.-SRBC (Sclavo, Siena, Italy) were washed twice with saline and 4 x 108 cells in 0-1 ml saline were injected i.p. 0-02 mg LPS in 0-1 ml saline was injected i.p. Detection of antibody producing cells.The number of direct haemolytic plaque forming cells (PFC) against SRBC was determined on Day 4 and 5 following immunization, according to Jerne, Nordin and Henry (1963). Statistical analysis.-Student's t test was used to compare the experimental results
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