Abstract
Objective To observe the effect of RNA interference to c-Myc on proliferation of gastric cancer SGC7901 cells in vitro and in vivo. Methods The c-Myc gene of gastric cancer SGC7901 was silenced by RNA interference technique. The expression of c-Myc gene of SGC7901 was detected by real-time fluorescent quantitative polymerase chain reaction (FQ-PCR). The proliferation of gastric cancer SGC7901 was detected by methyl thiazol tetrazolium (MTT) assay and in vivo tumorigenesis. Results After the silencing of c-Myc gene by RNA interference technology, the proliferation rate and tumorigenic growth rate of gastric cancer SGC7901 decreased significantly (on day 7, compared with the blank group, the tumor inhibition rate of small interfering RNA (siRNA)-scramble group was 98.78% (P>0.05), while that of the experimental group was 49.96% (P<0.05). Conclusion After the c-Myc interference, gastric cancer SGC7901 in vitro and in vivo proliferation rate decreased. Key words: Gastric carcinoma; RNA interference; c-Myc; Proliferation
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